DIPEPTIDYL PEPTIDASE-IV INHIBITORY ACTIVITY OF SOME INDONESIAN MEDICINAL PLANTS

ABSTRACT Objective: The focus of this research is to determine the inhibitory activity of ethanol extracts from 20 plants that have been used traditionally in Indonesia for the treatment of diabetes for dipeptidyl peptidase-IV (DPP-IV) inhibitory activity. Methods: A crude drug was extracted by reflux methods using 96% ethanol for 1 hr. The assay was performed with modification methods of Al Masri et al. using 96 micro well plates, and the absorbance was measured at 380 nm using the microplate reader. Sitagliptin was used as a standard inhibitor DPP-IV. Results: About 5 of 20 ethanol extracts showed the ability to inhibit DPP-IV activity in vitro at a concentration of 2.5 ug/ml. The active extract was obtained from pomegranates rind, bungur leaves, brotowali stem, bodhi leaves, and fenugreek seeds. The inhibitory percentage of the fifth extract was 58.79±2.23, 60.22±2.01, 65.86±0.02, 68.98±1.95, and 71.29±0.33. The IC50 value of sitagliptin was 1.104 ug/ml. Conclusion: The results of in vitro assay showed that some extract inhibited DPP-IV. The fenugreek seed showed the highest inhibition to DPP-IV. Keywords: Dipeptidyl peptidase-IV, Dipeptidyl peptidase-IV inhibitory, Fenugreek seed, Sitagliptin.