人巨细胞病毒
神经生长因子
污渍
生物
细胞培养
细胞病变效应
胶质瘤
体外
病毒学
巨细胞病毒
病毒
疱疹病毒科
基因
癌症研究
病毒性疾病
受体
生物化学
遗传学
作者
Haitao Wang,Bin Wang,Zhijun Liu,Zhiqiang Bai,Ling Li,Haiyan Liu,Dongmeng Qian,Yan Zhang,Xuejiao Song
标识
DOI:10.1016/s0895-3988(09)60068-4
摘要
To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV). U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV. The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P<0.05). HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.
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