神经酰胺
微泡
姜黄素
外体
细胞生物学
细胞内
微泡
生物化学
化学
生物
细胞凋亡
基因
小RNA
作者
David García-Seisdedos,Bohdan Babiy,Milagros Lerma,María E. Casado,Javier Martı́nez-Botas,Miguel A. Lasunción,Óscar Pastor,Rebeca Busto
标识
DOI:10.1016/j.bbalip.2020.158638
摘要
Curcumin, a hydrophobic polyphenol found in the rhizome of Curcuma longa, has been shown to reduce intracellular lipid accumulation in mouse models of lysosomal storage diseases such as Niemann-Pick type C. Exosomes are small extracellular vesicles secreted by cells in response to changes in intracellular ceramide composition. Curcumin can induce exosome/microvesicle release in cellular models of lipid deposition; however, the mechanism by which curcumin stimulates this release is unknown. In a model of lipid trafficking impairment in C6 glia cells, we show that curcumin stimulated ceramide synthesis by increasing the intracellular concentration of ceramide-dihydroceramide. Ceramide overload increased exosome/microvesicle secretion 10-fold, thereby reducing the concentration of lipids in the endolysosomal compartment. These effects were blocked by inhibitors of serine palmitoyltransferase (myriocin) and ceramide synthase (fumonisin B1). It is concluded that the decrease in intracellular lipid deposition induced by curcumin is mediated by increased ceramide synthesis and exosome/microvesicle release. This action may represent an additional health benefit of curcumin.
科研通智能强力驱动
Strongly Powered by AbleSci AI