清脆的
西斯特
核糖核酸
计算生物学
生物
引导RNA
RNA结合蛋白
Cas9
马拉特1
背景(考古学)
蛋白质-蛋白质相互作用
长非编码RNA
基因
遗传学
X-失活
X染色体
古生物学
作者
Wenkai Yi,Jingyu Li,Xiaoxuan Zhu,Xi Wang,Ligang Fan,Wenju Sun,Linbu Liao,Jilin Zhang,Xiaoyü Li,Jing Ye,Fulin Chen,Jussi Taipale,Kui Ming Chan,Liang Zhang,Jian Yan
出处
期刊:Nature Methods
[Springer Nature]
日期:2020-06-22
卷期号:17 (7): 685-688
被引量:83
标识
DOI:10.1038/s41592-020-0866-0
摘要
We have developed CRISPR-assisted RNA-protein interaction detection method (CARPID), which leverages CRISPR-CasRx-based RNA targeting and proximity labeling to identify binding proteins of specific long non-coding RNAs (lncRNAs) in the native cellular context. We applied CARPID to the nuclear lncRNA XIST, and it captured a list of known interacting proteins and multiple previously uncharacterized binding proteins. We generalized CARPID to explore binders of the lncRNAs DANCR and MALAT1, revealing the method's wide applicability in identifying RNA-binding proteins.
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