Neerizhivu kudineer (a traditional siddha polyherbal antidiabetic medicine) inhibits α- amylase enzyme and αglucosidase enzyme

Objective: To investigate the α-amylase and α-glucosidases inhibitory activity of Neerizhivu kudineer (NK). Methods: The polyherbal formulation NK was prepared as per traditional Siddha medical literature and aqueous extract was taken. Invitro α-amylase and α-glucosidases inhibitory activity of NK was evaluated using various concentrations of NK. Acarbose was used as standard drug. The percent inhibition values were determined and the dose verses percent inhibition was plotted in MS excel. Using the linear trend line, the concentration required for 50% inhibition (IC50 value) were calculated. Results: NK exhibited dose dependent inhibition on α- amylase and α-glucosidase with the IC50 value of 6.90 μg/ml and 8.51 μg/ml respectively, whereas the standard drug acarbose exhibited IC50 at 5.04 μg/ml and 5.50 μg/ml respectively. Conclusion: Neerizhivu kudineer has the inhibitory action on α-amylase and α-glucosidases enzyme.