球体
真皮成纤维细胞
体外
化学
生物医学工程
纳米技术
成纤维细胞
材料科学
工程类
生物化学
作者
Zhengkun Chen,Sina Kheiri,Albert Gevorkian,Elton T. Young,Valérie André,Ted Deisenroth,Eugenia Kumacheva
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2021-01-01
卷期号:21 (20): 3952-3962
被引量:16
摘要
Organotypic micrometre-size 3D aggregates of skin cells (multicellular spheroids) have emerged as a promising in vitro model that can be utilized as an alternative of animal models to test active ingredients (AIs) of skincare products; however, a reliable dermal spheroid-based microfluidic (MF) model with a goal of in vitro AI screening is yet to be developed. Here, we report a MF platform for the growth of massive arrays of dermal fibroblast spheroids (DFSs) in a biomimetic hydrogel under close-to-physiological flow conditions and with the capability of screening AIs for skincare products. The DFSs formed after two days of on-chip culture and, in a case study, were used in a time-efficient manner for screening the effect of vitamin C on the synthesis of collagen type I and fibronectin. The computational simulation showed that the uptake of vitamin C was dominated by the advection flux. The results of screening the benchmark AI, vitamin C, proved that DFSs can serve as a reliable in vitro dermal model. The proposed DFS-based MF platform offers a high screening capacity for AIs of skincare products, as well as drug discovery and development in dermatology.
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