Phage display : a practical approach

1. Introduction to phage biology and phage display 2. Constructing phage display libraries by oligonucleotide-directed mutagenesis 3. In vitro DNA recombination 4. Phage selection strategies for improved affinity and specificity of proteins and peptides 5. Rapid screening of phage displayed protein binding affinities by phage ELISA 6. Identification of novel ligands for receptors using recombinant peptide libraries 7. Substrate phage display 8. Protease-based selection of stably folded proteins and protein domains from phage display libraries 9. Phage display of zinc fingers and other nucleic acid-binding motifs 10. In vivo and ex vivo selections using phage-displayed libraries 11. Screening phage libraries with sera 12. Interaction cloning using cDNA libraries displayed on phage 13. Phage antibody libraries 14. Affinity maturation of phage antibodies