Amino-modified polystyrene nanoplastics induced multiple response of Artemia hemocytes

Polystyrene polymers cause severe toxicity to aquatic animals. However, the process and mechanisms of innate immunity of invertebrates living at the bottom of the food chain to these pollutants remain unclear. In this study, the blood system responses of zooplankton Artemia were assessed through in vivo and in vitro exposure to amino-modified polystyrene nanoplastics (PS-NH 2 NPs). The results indicated that the LC 50 values of PS-NH 2 NPs were 1.09 μg·mL −1 over 48 h and 0.42 μg·mL −1 over 7 d. Based on the five hemocyte subpopulations identified in Artemia , in vitro exposure assays revealed that phagocytosis was performed by plasmocytes and granulocytes with phagocytic rate of 22.64 %. TEM analysis further showed that PS-NH 2 NPs caused cytoplasm vacuolization, swollen mitochondria, and lipid processing disorder. Gene expression pattern results demonstrated that Spatzle, Tollip, Hsp70, Hsp90, Casp8, API5and Pxn were significantly upregulated upon acute and chronic exposure ( p < 0.05), while chronic exposure could induce significantly upregulation of ProPO ( p < 0.05). Moreover, PS-NH 2 NPs exposure remarkably varied the hemolymph microbiota and hemogram, particularly by increasing the proportion of adipohemocytes and phagocytes ( p < 0.05) . Our findings suggest that PS-NH 2 NPs induce different responses in Artemia hemocyte, as primarily reflected by phagocytic processes, expression of immune and apoptosis relating genes, cell fates, hemogram and hemolymph microbiota variations. These findings support the possibility of using Artemia hemocytes as bioindicator to estimate nanoplastics pollution, thus contributing to hematological toxicity research in response to nanoplastics. • We identified hemocyte populations and revealed hemogram variation of Artemia . • It is worth paying attention to the immunocyto-toxicity caused by PS-NH 2 NPs. • Significant changes of hemolymph microbiota occurred after chronic exposure. • Artemia hemocyte is a potential indicator to evaluate the toxicity of nanoplastics.