连接蛋白
缝隙连接
细胞生物学
LY294002型
MAPK/ERK通路
蛋白激酶B
颗粒细胞
生物
细胞内
胰岛素
卵泡发生
内科学
化学
卵泡期
内分泌学
信号转导
医学
胚胎
胚胎发生
作者
Lin Yin,Wusu Wang,Wei Pang,Gongshe Yang,Lei Gao,Guiyan Chu
标识
DOI:10.1016/j.theriogenology.2023.09.008
摘要
Gap junction intercellular communication (GJIC) among granulosa cells plays an important role in folliculogenesis, and it is temporal-spatially regulated during follicular development. Connexin (Cx) proteins predominantly form the basal structure of gap junctions in granulosa cells. In our study, immunohistochemical analysis revealed that Cx43 is the most widely expressed connexin in porcine follicles, especially among the large antral follicles. With application of insulin on porcine granulosa cells, we found that insulin significantly facilitated the protein level of Cx43, not mRNA level. This process is dependent on the phosphorylated activities of AKT and Erk since selective AKT and Erk inhibitors, LY294002 and U0126, respectively, hampered the potential of insulin to up-regulate Cx43 protein expression. As a consequence, the insulin-enhanced Cx43-couple GJIC activity in porcine granulosa cells was corresponding attenuated by the administration of LY294002 and U0126. Our findings provide a new insight into the molecular mechanisms by which insulin mediates cell-cell communication in porcine granulosa cells and sheds light on nutrition-reproduction interactions.
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