Probiotic with gluten reduction property and its encapsulation in synbiotic aloe vera gel-alginate capsules with banana powder as prebiotic

This study aims to hydrolyze the immunogenic gluten peptides by probiotic bacteria, Lactococcus lactis G01. It was isolated from curd and isolation was done based on ability to hydrolyze gluten. It was also tested for probiotic properties such as survival in gastric juice, bile salts, acid resistance, antibiotic sensitivity, antioxidant potential, sodium chloride tolerance, and antimicrobial activity. Lactococcus lactis G01 exhibited potential probiotic properties also, hence it was selected for microencapsulation. Probiotic was encapsulated in sodium alginate beads using banana powder as prebiotic and aloe vera as the adsorbent. The bead morphology was studied using scanning electron microscopy and transmission electron microscopy. The chemical composition of the bead was confirmed by FTIR. It was observed that 99% of the encapsulated probiotic cells were released into the simulated intestinal fluid in 90 min. Storage study was conducted for encapsulated probiotic and after four weeks of storage, the probiotic count in microcapsules was 7.82 log10 CFU/g. The formulated synbiotic capsules are suggested to incorporate in porridge for celiac patients since the probiotic has gluten reduction property.