Halotolerant Dunaliella viridis is the most salt-tolerant eukaryotes in the world by now,D.viridis contain many kinds of nutritional labeling and active materials of the living beings,such as plant albumen,fatty acid,polysaccharide,chlorophyll and glycerine and so on.It can apply to developing natural healthy products or biological medicines,so it has a bright future.This thesis discusses the effect that the different ratio of Na+/Mg2+,Cl-/SO2-4 to the growth of Halotolerant Dunlaliella viridis,and further analyzes the most suitable chemistry environment to the growth of Halotolerant Dunlaliella viridis according to the theory and method of the artificial sea water.