Determination of free gossypol in cottonseed cake feed by HPLC

The free gossypol in cottonseed cake feed by HPLC Free gossypol was immersed and extracted through micropore filter,and then analyzed by HPLC.The optimal conditions of HPLC were as follows: the column of chromatogram Sepax sapphire C185μm 120A(250 mm×4.6 mm),mobile phase acetonitrile/water(10mmol/L KH2PO4)=80/20,UV-238nm detector,column temperature 25 ℃,flow rate 1mL/min.The minimum detection limit of gossypol was determined to be 0.94 mg/kg,the average recoveries of the method were 96.23%～99.36%,and the RSD were 3.24%～4.02%.Compared with traditional chemical method,this method is reliable,simple,quick and accurate,an ideal method for determining the free gossypol in the cottonseed protein.