An experiment was conducted to assess the in vitro effect of spinosad 45 SC (Tracer ® ) on the entomopathogenic fungi viz., M. anisopliae and B. bassiana . The insecticide was applied at various concentrations and the field dose and higher doses adversely affected colony development, sporulation and spore germination. The effect was significantly higher on B. bassiana than on M. anisopliae and the effect increased with the dosage of insecticide used.