纤维素
细菌纤维素
ATP合酶
生物合成
生物化学
化学
细菌
聚合
细胞壁
负染色法
立体化学
聚合物
酶
电子显微镜
生物
有机化学
物理
光学
遗传学
作者
Mamoru Koyama,William Helbert,Tomoya Imai,Junji Sugiyama,Bernard Henrissat
标识
DOI:10.1073/pnas.94.17.9091
摘要
The "parallel-up" packing in cellulose Ialpha and Ibeta unit cells was experimentally demonstrated by a combination of direct-staining the reducing ends of cellulose chains and microdiffraction-tilting electron crystallographic analysis. Microdiffraction investigation of nascent bacterial cellulose microfibrils showed that the reducing end of the growing cellulose chains points away from the bacterium, and this provides direct evidence that polymerization by the cellulose synthase takes place at the nonreducing end of the growing cellulose chains. This mechanism is likely to be valid also for a number of processive glycosyltransferases such as chitin synthases, hyaluronan synthases, and proteins involved in the synthesis of nodulation factor backbones.
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