An ICT-based strategy to a colorimetric and ratiometric fluorescence probe for hydrogen sulfide in living cells

We present a colorimetric and ratiometric fluorescent probe Cy-N(3) that exhibits a selective response to H(2)S. The probe employs a near-infrared cyanine as a fluorophore, and is equipped with an operating azide unit. It is readily employed for assessing intracellular H(2)S levels, and confocal ratiometric imaging is achieved successfully.