Immunomodulatory effects of arborside C pentaacetate- an iridoid glycoside acetate isolated from Nyctanthes arbor-tristis Linn. and in vitro and in silico studies

Following a bio-assay guided fractionation and chromatographic separations a known compound arborside C pentaacetate was isolated. Immunomodulatory effects of arborside C pentaacetate were revealed by inhibition of NO, pro-inflammatory mediators, IL-6, TNF-α, and NF-κB at translational level in an in vitro study. The docking results revealed that arborside C pentaacetate bind strongly to the target protein with the docking score of -10.38 kcal/mol. The compound was non-toxic on normal human fibroblast (BJ) and THP-1 cells and, could be a potential therapeutic agent in inflammation driven diseases.