二向色玻璃
光学
显微镜
二向色滤光片
镜头(地质)
全内反射荧光显微镜
波长
全内反射
材料科学
反射(计算机编程)
千分尺
雷
显微镜
物理
计算机科学
程序设计语言
作者
Shuo Wang,Min Hu,Beini Sun,Tao Pang,Zhengfei Zhuang,Tongsheng Chen
标识
DOI:10.1002/jbio.202300190
摘要
The dichroic mirror (DM) is a key component in microscope. We found a ghost in the reflection channel of a dual-channel fluorescence microscope and studied the relationship between the ghost and the incidence angle θ into the DM. The DM emission surface reflection generated ghost if the θ is not 45° . We analyzed the distance and intensity relationship between the ghost and the primary image, which is θ -dependent and was demonstrated by imaging live cells and a stage micrometer. The ghost can be eliminated by placing the DM between objective and tube lens, but not between tube lens and detector, ensuring that the incident light into the DM is approximately parallel. Furthermore, the transmitted light of the DM is shifted towards a longer wavelength with increasing θ . Collectively, microscopists must carefully optimize the θ when designing a microscope to avoid the ghost.
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