类有机物
程序性细胞死亡
生物
细胞生物学
癌细胞
癌症
细胞凋亡
生物化学
遗传学
作者
Wenxin Li,Yujie Su,Jingyi Guo,Mengfei Wang,Xingguo Liu
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 117-133
标识
DOI:10.1007/978-1-0716-3433-2_11
摘要
Ferroptosis is a unique form of iron-dependent cell death induced by lipid peroxidation and subsequent plasma membrane rupture, which sets it apart from other types of regulated cell death. Ferroptosis has been linked to a diverse range of biological processes, such as aging, immunity, and cancer. Organoids, on the other hand, are three-dimensional (3D) miniaturized model systems of different organs in vitro cultures, which have gained widespread interest for modeling tissue development and disease, drug screening, and cell therapy. Organoids offer tremendous potential for improving our understanding of human diseases, particularly in the search for the field of ferroptosis in pathological processes of organs. Furthermore, cancer organoids are utilized to investigate molecular mechanisms and drug screening in vitro due to the anti-tumor effect of ferroptosis. Currently, the development of liver organoids has reached a relatively mature stage. Here, we present the protocols for the generation of liver organoids and liver cancer organoids, along with the methods for detecting ferroptosis in organoids.
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