普氏粪杆菌
生物
代谢组
转录组
代谢组学
脂肪肝
微生物群
脂肪变性
微生物学
小桶
疾病
生物信息学
遗传学
粪便
基因
医学
基因表达
病理
内分泌学
作者
María Florencia Mascardi,Flavia Noelia Mazzini,Bárbara Suárez,Vera M. Ruda,Sebastián Marciano,Paola Casciato,Adrián Narvaez,Leila Haddad,Margarita Anders,Federico Orozco,Ana Jesica Tamaroff,Frank Lauderdale Cook,John Gorcsan,Susana Gutt,Adrián Gadano,Celia Méndez García,Martı́n L. Marro,Alberto Penas Steinhardt,Julieta Trinks
出处
期刊:Proteomics
[Wiley]
日期:2023-07-31
卷期号:23 (18)
标识
DOI:10.1002/pmic.202200414
摘要
Interactions between communities of the gut microbiome and with the host could affect the onset and progression of metabolic associated fatty liver disease (MAFLD), and can be useful as new diagnostic and prognostic biomarkers. In this study, we performed a multi-omics approach to unravel gut microbiome signatures from 32 biopsy-proven patients (10 simple steatosis -SS- and 22 steatohepatitis -SH-) and 19 healthy volunteers (HV). Human and microbial transcripts were differentially identified between groups (MAFLD vs. HV/SH vs. SS), and analyzed for weighted correlation networks together with previously detected metabolites from the same set of samples. We observed that expression of Desulfobacteraceae bacterium, methanogenic archaea, Mushu phage, opportunistic pathogenic fungi Fusarium proliferatum and Candida sorbophila, protozoa Blastocystis spp. and Fonticula alba were upregulated in MAFLD and SH. Desulfobacteraceae bacterium and Mushu phage were hub species in the onset of MAFLD, whereas the activity of Fonticula alba, Faecalibacterium prausnitzii, and Mushu phage act as key regulators of the progression to SH. A combination of clinical, metabolomic, and transcriptomic parameters showed the highest predictive capacity for MAFLD and SH (AUC = 0.96). In conclusion, faecal microbiome markers from several community members contribute to the switch in signatures characteristic of MAFLD and its progression towards SH.
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