Hierarchical MXene/Enzyme@Covalent Organic Framework Composite by Template Method for Photothermal‐Enhanced Lipase Catalysis

Abstract Enzyme immobilization has emerged as a promising strategy to facilitate the industrialization of biomolecules. However, immobilized systems are now still facing great challenges such as maintaining enzyme activity, large‐scale manufacturing recovery and leaching or conformational changes of enzymes. Therefore, advanced enzyme immobilization techniques (efficient, stable and tunable catalysis) are currently an important challenge in this field. Here, enzyme@cyclodextrin covalent organic framework films are synthesized by template method and further constructed three‐dimensional (3D) hydrogen bond‐enhanced efficient MXene/enzyme membrane reactor. This strategy provides a spacious microenvironment for the release of enzyme molecules, a simpler process, an easier‐to‐etch template, a more biocompatible carrier. Furthermore, a versatile platform for interfacial photothermal and fast mass‐transfer by constructing films with MXene. The obtained biocatalysts have excellent reusability, high activity and stability, which can be used as efficient biocatalysts for important industrial reactions. The enzyme membrane reactor is constructed by the ‘smart capsule ’ with enzyme@cyclodextrin‐COF structure. The strategy can solve the challenging problems of the original previous enzyme immobilization system. Overall, a new idea is provided for the green, efficient, stable and sustainable general enzyme catalytic platform.