成熟
开始成熟
生物
单倍型
数量性状位点
连锁不平衡
遗传学
单核苷酸多态性
基因
基因型
植物
作者
Guang‐Qi He,Xi-Xi Huang,Mao-Song Pei,Hui‐Ying Jin,Yi‐Zhe Cheng,Tong-Lu Wei,Hai-Nan Liu,Yi‐He Yu,Da‐Long Guo
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2022-11-28
卷期号:191 (2): 1153-1166
被引量:1
标识
DOI:10.1093/plphys/kiac539
摘要
Pearl of Csaba (PC) is a valuable backbone parent for early-ripening grapevine (Vitis vinifera) breeding, from which many excellent early ripening varieties have been bred. However, the genetic basis of the stable inheritance of its early ripening trait remains largely unknown. Here, the pedigree, consisting of 40 varieties derived from PC, was re-sequenced for an average depth of ∼30×. Combined with the resequencing data of 24 other late-ripening varieties, 5,795,881 high-quality single nucleotide polymorphisms (SNPs) were identified following a strict filtering pipeline. The population genetic analysis showed that these varieties could be distinguished clearly, and the pedigree was characterized by lower nucleotide diversity and stronger linkage disequilibrium than the non-pedigree varieties. The conserved haplotypes (CHs) transmitted in the pedigree were obtained via identity-by-descent analysis. Subsequently, the key genomic segments were identified based on the combination analysis of haplotypes, selective signatures, known ripening-related quantitative trait loci (QTLs), and transcriptomic data. The results demonstrated that varieties with a superior haplotype, H1, significantly (one-way ANOVA, P < 0.001) exhibited early grapevine berry development. Further analyses indicated that H1 encompassed VIT_16s0039g00720 encoding a folate/biopterin transporter protein (VvFBT) with a missense mutation. VvFBT was specifically and highly expressed during grapevine berry development, particularly at veraison. Exogenous folate treatment advanced the veraison of "Kyoho". This work uncovered core haplotypes and genomic segments related to the early ripening trait of PC and provided an important reference for the molecular breeding of early-ripening grapevine varieties.
科研通智能强力驱动
Strongly Powered by AbleSci AI