Bimetallic silver-platinum (AgPt) nanoparticles and chitosan fabricated cotton gauze for enhanced antimicrobial and wound healing applications

Wound healing is a significant clinical and socioeconomic problem that is often affected by microbial infection. Inappropriate monitoring leads to unfavorable concerns for surrounding tissues. Cotton gauzes have been used as low-cost wound dressing material but prolong healing owing to strong adherence and secondary microbial infections. Hence, we prepared the bimetallic (silver and platinum) nanoparticles (AgPt NPs) using citric acid (CA) as a reducing agent and then coated them on chitosan (CS) fabricated cotton gauze (CG) for enhanced antimicrobial and wound healing applications. The synthesis of AgPt NPs was evidenced UV-Visible spectroscopy, FE-TEM, and elemental mapping analysis. The average size of AgPt NPs was 21.48 ± 6.32 nm and spherical in structure. Besides, AgPt NPs showed a hydrodynamic size of 63.64 (d.nm) with a polydispersity index of 0.220 and a zeta potential of -28.1 mV. The FT-IR and XRD analysis demonstrated the functional changes and crystalline properties of AgPt NPs. The antimicrobial efficacy of AgPt NPs was significantly higher than standard antibiotic against bacteria, yeast, and filamentous fungi. Furthermore, the AgPt NPs-CS/CG exhibited a substantial hydrophobic nature with better antimicrobial and antioxidant activity. In addition, pH-dependent Ag and Pt release from the AgPt NPs-CS/CG was determined by ICP-MS analysis. The treatment of AgPt NPs-CS/CG augmented the in vitro wound healing in mouse embryonic fibroblast cells (NIH3T3). Hence, we concluded that AgPt NPs-CS/CG could be used to enhance antimicrobial and wound healing applications.