生物传感器
葡萄糖氧化酶
检出限
材料科学
循环伏安法
介电谱
计时安培法
纳米技术
基质(水族馆)
化学
色谱法
电化学
电极
地质学
海洋学
物理化学
作者
R. Bi,Xiaoyan Ma,Kunpeng Miao,Pengcheng Ma,Qianqian Wang
标识
DOI:10.1016/j.enzmictec.2022.110132
摘要
With the development of blood glucose monitoring medical devices, higher requirements are put forward for the sensitivity and detection limit of biosensors. Herein, a high-performance flexible enzymatic glucose biosensor was prepared by electrochemical deposition of dendritic gold nanostructure on carbon cloth substrate, then covalently immobilizing glucose oxidase on the dendritic gold, and then increasing enzyme loading by enzyme precipitation coating. The morphology of dendritic gold nanostructures was characterized by scanning electron microscopy (SEM). The electrochemical performance of the biosensor was analyzed by cyclic voltammetry (CV), chronoamperometry curve and electrochemical impedance spectroscopy (EIS). It is found that the obtained biosensor has high sensitivity (72.45 μA mM-1 cm-2), low detection limit (6.7 μM) and ultra-wide linear range (0.02-31.7 mM) for glucose detection. In addition, the biosensor also has good selectivity, reproducibility and stability. In the detection of real serum samples, the biosensor also shows good accuracy and practicality. The results show that the new biosensor has great potential for applications in biomedical and health care.
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