支持细胞
褪黑素
内科学
内分泌学
缺氧(环境)
纤维化
精子发生
生物
医学
化学
有机化学
氧气
作者
Huawei Wei,Longfei Xiao,Hua Yao,Xiang Li,Wei Wang,Yuan Lee,Dongyan Li,Jingwen Wei
标识
DOI:10.1016/j.repbio.2022.100677
摘要
The hypoxic microenvironment of cryptorchidism is an important factor in the impairment and fibrosis of Sertoli cells which result in blood-testis barrier (BTB) destruction and spermatogenesis loss. Recent studies have shown that melatonin , a well-known pineal hormone exerts beneficial effects against pathological fibrosis in a various of organs. However, it is still unknown whether melatonin can regulate hypoxia-induced fibrosis of Sertoli cells. In this study we evaluate melatonin levels, and its synthesizing enzymes, AANAT and HIOMT expression patterns in canine cryptorchidism and contralateral normal testis . Results show abdominal testes presented low melatonin levels and AANAT and HIOMT expression compared with testes located in the scrotum . Moreover, we established a hypoxia-induced fibrosis model in canine Sertoli cells induced by cobalt chloride (CoCl 2 ) and found that melatonin inhibited the EMT markers expression and ECM production as well as Hif-1α expression of Sertoli cells in a dose-dependent manner. Furthermore, use of Lificiguat (synonyms YC-1, Hif-1α inhibitor) to interfere with the Hif-1α pathway showed a similar effect with melatonin suppression of the fibrosis in Sertoli cells. The results indicate that melatonin supplementation can alleviate the fibrosis process of Sertoli cells caused by hypoxia , which is associated with regulating the inhibition of Hif-1α signaling.
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