医学
转移
癌症研究
肿瘤微环境
生物发光成像
流式细胞术
免疫系统
细胞
肿瘤进展
病理
癌症
免疫学
生物
细胞培养
荧光素酶
内科学
转染
遗传学
作者
Danyi Zhai,Jing Huang,Yan Hu,Chao Wan,Yajie Sun,Jingshu Meng,Huaduan Zi,Lisen Lu,Qianyuan He,Yu Hu,Jin Huang,Kunyu Yang
标识
DOI:10.1016/j.ijrobp.2022.06.092
摘要
The majority of cancer-related deaths are attributed to metastasis rather than localized primary tumor progression. However, the factors that regulate the premetastatic niche (PMN) and metastasis have not yet been clearly elucidated. We investigated the antimetastatic effects of irradiated tumor cell-derived microparticles (RT-MPs) and highlighted the role of innate immune cells in PMN formation.Mice were treated 3 times with isolated RT-MPs, followed by tumor cell injection via the tail vein. The hematoxylin and eosin staining was performed to assess the number of tumor nodules in the lungs, and in vivo luciferase-based noninvasive bioluminescence imaging was conducted to detected tumor burden. The mechanisms of RT-MPs mediated PMN formation was evaluated using flow cytometry, transwell assay, and reverse transcription-polymerase chain reaction.RT-MPs inhibited tumor cell colonization in the lungs. Neutrophils phagocytosed RT-MPs and secreted CCL3 and CCL4, which induced monocytes chemotaxis and maturation into macrophages. RT-MPs promoted the transition of neutrophils and macrophages into antitumor phenotypes, hence inhibiting cancer cell colonization and proliferation.RT-MPs inhibited PMN formation and lung metastasis in a neutrophil- and macrophage-dependent but T cell-independent manner.
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