间皮
间皮细胞
细胞生物学
类有机物
生物
细胞外基质
干细胞
诱导多能干细胞
细胞培养
Wnt信号通路
胚胎干细胞
病理
信号转导
遗传学
医学
基因
作者
Meghan M. Capeling,Sha Huang,Charlie J. Childs,Joshua H. Wu,Yu-Hwai Tsai,Angeline Wu,N. Garg,Emily M. Holloway,Nambirajan Sundaram,Carine Bouffi,Michael A. Helmrath,Jason R. Spence
出处
期刊:Cell Reports
[Elsevier]
日期:2022-02-01
卷期号:38 (7): 110379-110379
被引量:16
标识
DOI:10.1016/j.celrep.2022.110379
摘要
Pluripotent-stem-cell-derived human intestinal organoids (HIOs) model some aspects of intestinal development and disease, but current culture methods do not fully recapitulate the diverse cell types and complex organization of the human intestine and are reliant on 3D extracellular matrix or hydrogel systems, which limit experimental control and translational potential for regenerative medicine. We describe suspension culture as a simple, low-maintenance method for culturing HIOs and for promoting in vitro differentiation of an organized serosal mesothelial layer that is similar to primary human intestinal serosal mesothelium based on single-cell RNA sequencing and histological analysis. Functionally, HIO serosal mesothelium has the capacity to differentiate into smooth-muscle-like cells and exhibits fibrinolytic activity. An inhibitor screen identifies Hedgehog and WNT signaling as regulators of human serosal mesothelial differentiation. Collectively, suspension HIOs represent a three-dimensional model to study the human serosal mesothelium.
科研通智能强力驱动
Strongly Powered by AbleSci AI