Simultaneous determination of aflatoxins, fumonisin B1, T-2 and cyclopiazonic acid in agri-products by immunomagnetic solid-phase extraction coupled with UHPLC-MS/MS

In the present study, a rapid and sensitive determination method of seven mycotoxins was developed using immunomagnetic solid-phase extraction (IMPSE) coupled with UHPLC-MS/MS. Monoclonal antibodies were conjugated with CNBr superparamagnetic beads, and the major parameters affecting the IMPSE efficiency were systematically investigated. Under the optimized conditions, the mycotoxins were purified with the IMSPE procedure within 15 min and simultaneously quantified by UHPLC-MS/MS. Good linearities of the analytic method were established with the determination coefficients (R2) ranging from 0.9952 to 0.9997. The limit of quantifications were 0.04 µg kg-1 ∼ 0.16 µg kg-1, which fully satisfied the regulatory maximum levels. The recoveries were 84.5-112.7% with intra-day and inter-day precision less than 15.2%. Finally, the proposed IMSPE-UHPLC-MS/MS was successfully utilized to analyze seven mycotoxins in peanut, maize, and wheat, providing a simple and robust extraction and enrichment procedure of hydrophilic and zwitterionic analytes from complex matrix.