烷烃
烷基苯
碳氢化合物
烷基
化学
产甲烷
富集培养
古细菌
产甲烷菌
生物化学
环境化学
有机化学
甲烷
立体化学
细菌
生物
基因
催化作用
遗传学
作者
Zhuo Zhou,Cui-Jing Zhang,Pengfei Liu,Лин Фу,Rafael Laso-Pérez,Yang Lü,Liping Bai,Li Jiang,Min Yang,Jietao Lin,Weidong Wang,Gunter Wegener,Meng Li,Lei Cheng
出处
期刊:Nature
[Springer Nature]
日期:2021-12-22
卷期号:601 (7892): 257-262
被引量:81
标识
DOI:10.1038/s41586-021-04235-2
摘要
The methanogenic degradation of oil hydrocarbons can proceed through syntrophic partnerships of hydrocarbon-degrading bacteria and methanogenic archaea1-3. However, recent culture-independent studies have suggested that the archaeon 'Candidatus Methanoliparum' alone can combine the degradation of long-chain alkanes with methanogenesis4,5. Here we cultured Ca. Methanoliparum from a subsurface oil reservoir. Molecular analyses revealed that Ca. Methanoliparum contains and overexpresses genes encoding alkyl-coenzyme M reductases and methyl-coenzyme M reductases, the marker genes for archaeal multicarbon alkane and methane metabolism. Incubation experiments with different substrates and mass spectrometric detection of coenzyme-M-bound intermediates confirm that Ca. Methanoliparum thrives not only on a variety of long-chain alkanes, but also on n-alkylcyclohexanes and n-alkylbenzenes with long n-alkyl (C≥13) moieties. By contrast, short-chain alkanes (such as ethane to octane) or aromatics with short alkyl chains (C≤12) were not consumed. The wide distribution of Ca. Methanoliparum4-6 in oil-rich environments indicates that this alkylotrophic methanogen may have a crucial role in the transformation of hydrocarbons into methane.
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