兴奋性突触后电位
抑制性突触后电位
神经科学
生物
突触后电位
谷氨酸的
生物神经网络
爆裂
神经网络
光遗传学
体内
谷氨酸受体
受体
生物化学
生物技术
作者
Yan Cui,Kaiwen Kam,David Sherman,Wiktor A. Janczewski,Yu Zheng,Jack L. Feldman
出处
期刊:Neuron
[Elsevier]
日期:2016-08-01
卷期号:91 (3): 602-614
被引量:124
标识
DOI:10.1016/j.neuron.2016.07.003
摘要
Normal breathing in rodents requires activity of glutamatergic Dbx1-derived (Dbx1(+)) preBötzinger Complex (preBötC) neurons expressing somatostatin (SST). We combined in vivo optogenetic and pharmacological perturbations to elucidate the functional roles of these neurons in breathing. In transgenic adult mice expressing channelrhodopsin (ChR2) in Dbx1(+) neurons, photoresponsive preBötC neurons had preinspiratory or inspiratory firing patterns associated with excitatory effects on burst timing and pattern. In transgenic adult mice expressing ChR2 in SST(+) neurons, photoresponsive preBötC neurons had inspiratory or postinspiratory firing patterns associated with excitatory responses on pattern or inhibitory responses that were largely eliminated by blocking synaptic inhibition within preBötC or by local viral infection limiting ChR2 expression to preBötC SST(+) neurons. We conclude that: (1) preinspiratory preBötC Dbx1(+) neurons are rhythmogenic, (2) inspiratory preBötC Dbx1(+) and SST(+) neurons primarily act to pattern respiratory motor output, and (3) SST(+)-neuron-mediated pathways and postsynaptic inhibition within preBötC modulate breathing pattern.
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