血管平滑肌
细胞生物学
生物
表型
调节器
小RNA
血小板源性生长因子受体
关贸总协定
肌球蛋白
转录因子
细胞生长
心肌细胞
表型转换
壁细胞
生长因子
遗传学
内分泌学
平滑肌
基因
受体
作者
Clarice Gareri,Claudio Iaconetti,Sabato Sorrentino,C. Covello,Salvatore De Rosa,Ciro Indolfi
标识
DOI:10.1016/j.jmb.2017.05.008
摘要
MicroRNAs are key regulators of vascular smooth muscle cells (VSMCs) phenotypic switch, one of the main events responsible for bare metal in-stent restenosis after percutaneous coronary intervention. miR-125a-5p is an important modulator of differentiation, proliferation, and migration in different cell types; however, its role in VSMCs is still unknown. The aim of this study was to evaluate the role of miR-125a-5p in VSMCs phenotypic switch. Our results suggest that miR-125a-5p is highly expressed in VSMCs, but it is down-regulated after vascular injury in vivo. Its overexpression is sufficient to reduce VSMCs proliferation and migration, and it is able to promote the expression of selective VSMCs markers such as alpha smooth muscle actin, myosin heavy chain 11, and smooth muscle 22 alpha. Interestingly, miR-125a-5p directly targets ETS-1, a transcription factor implicated in cell proliferation and migration and is crucial in PDGF-BB pathway in VSMCs. Thus, miR-125a-5p in this context inhibits PDGF-BB pathway and is therefore a potential regulator of VSMCs phenotypic switch.
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