检出限
化学
免疫分析
荧光
色谱法
分析物
超氧化物歧化酶
高效液相色谱法
线性范围
单克隆抗体
分析化学(期刊)
抗体
生物化学
酶
物理
量子力学
免疫学
生物
作者
Xiaoming Yang,Yao Dou,Song Zhu
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2013-01-01
卷期号:138 (11): 3246-3246
被引量:5
摘要
Herein, a novel highly sensitive enhanced-fluorescence immunoassay for detection of superoxide dismutase (SOD) is established by combining surface-enhanced fluorescence (SEF) with immuno-magnetic separation. Based on a sandwich-type immunoassay, analytes in samples are first captured by magnetic beads coated with a monoclonal antibody and then “sandwiched” by another monoclonal antibody on silver nanoparticles labeled with fluorescein-labeled oligonucleotides in the presence of a magnet. Subsequently, the immune complex is enriched by exposure to a magnetic field. Lastly, the fluorescence intensity is measured according to the number of dissociated fluoresceins. The increased fluorescence intensity permits highly sensitive detection of SOD in a linear range of 10–8 × 105 pg mL−1, with a detection limit of 4 pg mL−1 at a signal-to-noise ratio of 3. Significantly, this method was validated for detection of SOD in human serum, human urine, and cosmetic samples. Moreover, the reliability and accuracy of results obtained by the enhanced-fluorescence method was confirmed by the analysis of high performance liquid chromatography (HPLC).
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