抗坏血酸
化学
尿酸
试剂
过氧化氢
猝灭(荧光)
激进的
抗氧化剂
过氧化物酶
色谱法
生物化学
有机化学
酶
荧光
食品科学
物理
量子力学
作者
Yuzo Kayamori,Yoshiaki Katayama,Takeyoshi Urata
标识
DOI:10.1016/s0009-9120(99)00082-x
摘要
Ascorbic acid interferes significantly in the oxidative reaction of chromogenic reagents by peroxidase and hydrogen peroxide. Currently, ascorbate oxidase is commonly utilized for eliminating the interference of ascorbic acid in the oxidative colorimetric reaction. This enzyme, however, displays several disadvantages, such as high cost, variation from lot to lot, and low stability. We applied a series of commercially available and stable radicals (ascorbic acid quenchers [AAQs]) for nonenzymatic quenching of ascorbic acid in the uricase-based uric acid determination in serum and urine.In order to evaluate the quenching activity of AAQs, a commercially available uric acid detection kit was used. TBA-80FR.NEO biochemical analyzer was utilized for the assay.4-Hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy free radical (AAQ-2) was the most effective ascorbic acid quencher among the four stable radicals, and the uric acid assay suffered no interference by AAQ-2. The ascorbic acid quenching ability of 2 mmol/L of AAQ-2 in reagent solution (reagent-I) was > or = 2 U/ml ascorbate oxidase in reagent solution.AAQ-2 was proven to be a suitable quencher of ascorbic acid in clinical samples.
科研通智能强力驱动
Strongly Powered by AbleSci AI