Device of anoxic chamber system and repair of potentially lethal damage of anoxic cells in vitro.

KANO, E., FURUKAWA, M., KANEKO, I., YOSHIKAWA, S., TSUBOUCHI, S., KONDO, T. and YAMAGATA, K. Device of Anoxic Chamber System and Repair of Potentially Lethal Damage of Anoxic Cells in Vitro. Tohoku J. exp. Med., 1986, 148 (1), 1-13-In order to study radiation responses of anoxic plateau phase cells in vitro, an air-tight anoxic chamber was devised in which Chinese hamster V-79 or Swiss mouse NIH3T3 cells were deoxygenated by replacing the air with saturatedly humidified N2 gas. The cells as adhered on the culture plate, which was contained in the anoxic chamber, were x-irradiated. The deoxygenated condition in the anoxic chamber was maintained as long as necessary. Oxygen enhancement ratio obtained by the anoxic chamber was about 2.6. Repair of potentially lethal damage of the anoxic plateau phase cells was observed to the same extent of that of the oxic cells.