Sequence and phylogenetic analysis of Beauveria bassiana with mitochondrial genome

Mitochondria are important cytoplasmic organelles of eukaryotic cells in energy metabolism and cell aging.Gene sequences of mitochondrial DNA (mtDNA) are also an important candidate for phylogenetical study.In the present paper,the whole mitochondrial DNA sequence of Beauveria bassiana,registered in EMBL with the accession number EU371503,was used to clarify the relationship between B.bassiana and 13 fungal species in 11 genera including Lecanicillium,Metarhizium,Hypocrea,Fusarium,Gibberella,Verticillium,Neurospora,Podospora,Penicillium,Aspergillus,and Trichophyton of Pezizomycotina.The result showed that rnl,rns,25 tRNAs and 14 protein genes of B.bassiana were all encoded by the sequences on the same strand and the most of the tRNAs arranged in three conserved trn gene clusters,revealing similarities to other members of Sordariomycetes.Phylogenetic analysis demonstrated that B.bassiana was highly similar to Lecanicillium muscarium based on the whole mtDNA sequence with querage coverage at 73%,and on 14 protein-coding genes with 100% bootstrap support by both NJ method and MP method.