ISSR fingerprint analysis of wild strains of Auricularia auricula in Heilongjiang Province.

In order to study the genetic relationship between the wild Auricularia auriculars in Heilongjiang Province,the paper used ISSR molecular markers to analyze 24 wild Auricularia auriculars of various regions of Heilongjiang Province and DNA finger-print of one cultivation Auricularia auriculars strain. To use NTSYS software conduct the cluster analysis on the basis of ISSR and divided 25 tested Auricularia auriculars strains into four groups under the similarity level of 0.49. Experimental result showed that the genetic relationship of wild Auricularia auriculars strains in Heilongjiang Province distribution apparently according to regional difference,genetic backgrounds in different areas had a certain difference and had a close similarity within the same region. But a few fungus strains which had adjacent producing area showed significant genetic relationship differences and the similarity between cultivated strains and wild strains had a large difference. Studies showed that,ISSR could be effectively used for identify the wild Auricularia auriculars strains fatly and accurately. It was not only an ideal way to study the Auricularia auriculars' finger-print but also provided the basis for the analysis of the wild Auricularia auriculars.