三角藻
牡荆素
异牡荆素
东方
传统医学
三角线
电喷雾电离
三级四极质谱仪
化学
质谱法
高效液相色谱法
电喷雾
串联质谱法
选择性反应监测
色谱法
医学
食品科学
类黄酮
有机化学
抗氧化剂
作者
Pratibha Singh,Vikas Bajpai,Vikaskumar Gond,Ashok Kumar,Tadigoppula Narender,Brijesh Kumar
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 377-393
被引量:16
标识
DOI:10.1007/978-1-0716-0235-5_21
摘要
Trigonella foenum-graecum (fenugreek), a legume has been used as spice throughout the world to enhance the sensory quality of foods, is considered to have a high nutraceutical value. The antidiabetic actions of Trigonella seeds have been speculated due to the presence of steroid saponins, alkaloids, and fiber content. Five bioactive compounds, namely, trigonelline, isoorientin, orientin, vitexin, and isovitexin, were detected and quantified by HPLC system. Further rapid detection of bioactive compounds was achieved using high-performance liquid chromatography hybrid electrospray quadrupole time-of-flight mass spectrometric (HPLC-ESI-QTOF-MS/MS) method. Twenty-five compounds were tentatively identified based on their retention time and measured accurate mass. The fragmentation patterns of known compounds were utilized to elucidate their structures. Six of these identified compounds, that is, 4-hydroxyisoleucine, trigonelline, isoorientin, isovitexin, pinitol, and sarsasapogenin, were simultaneously quantified by UHPLC-ESI-MS/MS method under the multiple reactions monitoring (MRM) mode using ultrahigh-performance liquid chromatography hybrid electrospray triple quadrupole linear ion trap mass spectrometer. The analytical method was validated and successfully applied for simultaneous determination of compounds in Fenugreek seeds, which indicated the suitability of the validated method for its quality control.
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