钆
纳米颗粒
磁共振造影剂
磁共振成像
铁蛋白
核磁共振
化学
材料科学
医学
纳米技术
生物化学
物理
放射科
有机化学
作者
Yong Cai,Yuqing Wang,Tongwei Zhang,Yongxin Pan
出处
期刊:ACS applied nano materials
[American Chemical Society]
日期:2020-08-11
卷期号:3 (9): 8771-8783
被引量:26
标识
DOI:10.1021/acsanm.0c01563
摘要
Recombinant human heavy chain (H-chain) ferritin (HFn) with gadolinium labeling (HFn-Gd) was designed as a targeted T1 contrast agent for magnetic resonance imaging (MRI) to enhance MRI efficiency in diagnosing tumors at a low dosage. The HFn-Gd nanoparticle shows a larger hydrodynamic diameter (13.6 nm) as compared to HFn (12.5 nm), and a decreased ζ potential value (−14.3 mv) as compared to HFn (−6.2 mv). Fourier transform infrared spectroscopy characterization and X-ray photoelectron spectroscopy analysis indicate the conjugation of Gd on HFn nanoparticles. Compared with Gd-DTPA (DTPA, diethylenetriaminepentaacetic acid), the HFn-Gd nanoparticle has a high r1 value of 4.78 mM–1 s–1, showing good biocompatibility, stability, and active targeting ability to TfR1-positive cancer cells as revealed by cell viability tests, arsenazo III colorimetric assays, and laser scanning confocal microscopy examinations. The MRI of CFPAC-1 tumors using HFn-Gd nanoparticles (dose = 0.016 mmol Gd/kg body weight) shows an enhanced signal up to 30 min postinjection, which is significantly longer than that of Gd-DTPA (approximately 10 min). At such a low dose, the MRI of microscopic MDA-MB-231 tumors, with HFn-Gd nanoparticles, shows a significant signal change up to 60 min, while almost no detectable signal change occurs with Gd-DTPA. Furthermore, MRI reveals that HFn-Gd nanoparticles are mostly cleared through the renal system. Our results demonstrate that the HFn protein-based contrast agent integrates T1 signal enhancing, precise tumor targeting, and systematic clearance capabilities. It is an effective T1 contrast agent for MR imaging of tumors with a single low-dose injection.
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