生物
间质细胞
细胞生物学
线粒体
细胞内
细胞周期
柠檬酸循环
三磷酸腺苷
内质网
氧化磷酸化
内分泌学
生物化学
新陈代谢
细胞凋亡
癌症研究
作者
Mi Jin Ryu,Bong Jong Seo,Yun Jeong Choi,Min Ji Han,Yun‐Sang Choi,Myung-Jin Chung,Jeong Tae
出处
期刊:Stem Cells and Development
[Mary Ann Liebert]
日期:2020-11-01
卷期号:29 (21): 1407-1415
被引量:6
标识
DOI:10.1089/scd.2020.0130
摘要
The endometrial cycle in response to hormonal stimulation is essential for implantation. The female has endometrium that repeats this cycle through about half of a lifetime. The cycle includes three phases, proliferative, secretory, and menstrual, and each phase has distinct characteristics. The endometrial stromal cells (EnSCs) in each phase also have specialized characteristics, including cell cycle, morphologies, and cellular metabolic state. So we hypothesized that the cells in each phase have unique mitochondrial morphologies because they are generally linked to cellular metabolic state. To investigate the metabolic characteristics in each phase, we investigated the mitochondrial morphologies by transmission electron microscopy, oxygen consumption rate (OCR), and intracellular adenosine triphosphate (ATP) production. The decidualized EnSCs have shorter mitochondria than those in the proliferative phase. Besides, they also displayed distinct intracellular structural characteristics compared with the proliferative phase, such as ribosome-rich endoplasmic reticulum and increased formation of vesicles. OCR and luminescent ATP detection assay revealed that the basal respiration and ATP production in the decidualized EnSCs were lower than those in the proliferative phase. Thus, we concluded that morphological and intracellular structural changes were induced during the decidualization. Moreover, the decreased mitochondrial length was shown to correlate with decreased dependency on oxidative phosphorylation and ATP concentration in EnSCs.
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