点头
信号转导
细胞生物学
细胞凋亡
炎症
下调和上调
矽肺
化学
巨噬细胞极化
NF-κB
线粒体
炎症体
巨噬细胞
生物
免疫学
医学
生物化学
基因
病理
体外
作者
Rong Fu,Qian Li,Rong Fan,Zhou Qinye,Xiaojuan Jin,Jin Cao,Jiabao Wang,Yongqiang Ma,Tailong Yi,Maobin Zhou,Sanqiao Yao,Hongsheng Gao,Zhongwei Xu,Zhen Yang
标识
DOI:10.1016/j.etap.2018.08.010
摘要
Silicosis is characterized by inflammation and pulmonary fibrosis due to long-term inhalation of crystalline silica (SiO2). To clarify the role of macrophage polarization in the inflammatory response of silicosis, we used iTRAQ-coupled 2D LC–MS/MS to study the change in the secretome in RAW264.7 macrophages. We successfully screened 330 differentially expressed proteins, including 120 proteins with upregulated expression and 210 proteins with down-regulated expression (p < 0.05). Bioinformatics analysis showed that the differentially expressed proteins were mainly involved in biological processes, such as oxidative stress, mitochondrial damage, apoptosis and acute inflammatory response. In particular, the expression levels of mitochondrial apoptosis-related proteins, such as AKT1, BAX, HSPD1, TNF, CASP8 and DAP, were increased after SiO2 exposure. Taken together, our study indicated that SiO2 could induce macrophage polarization by activation of the NOD-RIP2-NF-κB signaling pathway in RAW264.7 macrophages. This may represent a potential mechanism in the development of silicosis.
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