Abstract Here, we describe the using of biosynthesis method for the synthesis of Fe 3 O 4 , Cu nanoparticles and Fe 3 O 4 /Cu nanocomposite using Carum carvi extract and assessment of their antibacterial activity. Our approach is eco‐friendly, non‐toxic and clean. These nanoparticles/nanocomposites were characterized by UV‐Vis, FTIR, XRD, EDX and SEM, techniques. The findings indicated that the of Fe 3 O 4 and Cu NPs, and Fe 3 O 4 /Cu nanocomposite have an average particle size of 25 nm, 37 nm, and 62 nm, respectively. On the other side, the Fe 3 O 4 NPs do not have the antibacterial activity, but when they combine with Cu NPs with antibacterial activity, the Fe 3 O 4 /Cu nanocomposite shows the antimicrobial activity.