胡萝卜素
酿酒酵母
代谢工程
团块斑尾藻
酶
生物化学
甲戊酸途径
还原酶
食品科学
化学
酵母
生物
细菌
遗传学
作者
Beibei Wang,Mingyu Shi,Dong Wang,Jiaoyang Xu,Yi Liu,Hongjiang Yang,Zhaoli Dai,Xueli Zhang
出处
期刊:PubMed
日期:2014-08-01
卷期号:30 (8): 1204-16
摘要
β-carotene has a wide range of application in food, pharmaceutical and cosmetic industries. For microbial production of β-carotene in Saccharomyces cerevisiae, the supply of geranylgeranyl diphosphate (GGPP) was firstly increased in S. cerevisiae BY4742 to obtain strain BY4742-T2 through over-expressing truncated 3-hydroxy-3-methylglutaryl-CoA reductase (tHMGR), which is the major rate-limiting enzyme in the mevalonate (MVA) pathway, and GGPP synthase (GGPS), which is a key enzyme in the diterpenoid synthetic pathway. The β-carotene synthetic genes of Pantoea agglomerans and Xanthophyllomyces dendrorhous were further integrated into strain BY4742-T2 for comparing β-carotene production. Over-expression of tHMGR and GGPS genes led to 26.0-fold increase of β-carotene production. In addition, genes from X. dendrorhous was more efficient than those from P. agglomerans for β-carotene production in S. cerevisiae. Strain BW02 was obtained which produced 1.56 mg/g (dry cell weight) β-carotene, which could be used further for constructing cell factories for β-carotene production.
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