Identification of Geotrichum by RAPD

Objective To study the random amplified polymorphic DNA for rapid identification of Geotrichum.Methods DNAs of Geotrichum candidum,Geotrichum silvicola isolated from the skin lesion and blood were extracted by E.Z.N.A.yeast DNA kit and amplified using AP3(5'-TCGTAGCCAA-3'),ATG(5'-ATGGATCGGC-3'),RP2(5'-AAGGATCAGA-3'),OPA-10(5'-GTGATCGCAG-3')as primers.The characteristics of fingerprint was analyzed.Results DNA of Geotrichum was successfully extracted with satisfied purity and concentration for PCR.Distinct DNA band patterns showed differences in different Geotrichum species while displayed similarity within different strains of same species isolated from different lesions.Conclusions DNA extracted by E.Z.N.A.yeast DNA kit could applied for PCR.RAPD method is specific,rapid and simple for Geotrichum indetification.