Expression and clinical significance of WT1 gene in multiple myeloma

Objective To explore the expression and clinical significance of WT1 gene in multiple myeloma(MM).Methods The expression of WT1 gene was measured in the peripheral blood cells or bone marrow cells,of 25 patients with multiple myeloma and 10 normal controls by reverse transcriptase polymerase chain reaction(RT-PCR) method.Results WT1 gene was not detected in 10 normal controls.The positive expression rate of the WT1 gene in 25 patients was 36%.According to Durie-Salmon stage,WT1 gene was not detected in these patients with MM who was in the first phase of Durie-Salmon stage.But the expression of WT1 gene was higher in these patients of the second and third stage.The positive rates were 24%,63.64%,respectively.The positive rate of the expression of WT1 gene with ineffective treatment patients was 57.14%,the expression of WT1 gene in MM presented obvious stage by Durie-Salmon stage.The relative quantity of WT1 gene and the level of β2-microglobulin in Durie-Salmon stage of MM patients all had statistical significance compared between every two group(P0.05).They had a positive relationship between WT1 gene and the level of β2-microglobulin by correlation analysis,and related to the Durie-Salmon stage.The poor prognosis of patients was associated with the expression of WT1 gene after dynamic observation in 2 cases of patients with MM Ⅲ period.Conclusion The expression of WT1 gene could be an additional marker in risk assessment for MM.