Barley is one of the major cereal crops in the world.Due to its diploid nature,barley can be considered an important material for Triticeae genomic studies.With the application of techniques and methods employed in genomics,such as a large number of mapped molecular markers,BAC libraries,mutant collections and DNA arrays,the sequencing of barley genome has deepen increasingly.More and more information about the barley genome pave the way for a comprehensive analysis of structural and functional genomics of barley as well as understanding of gene expression networks linked to agronomically important traits.Progress in genomics and molecular breeding of barley were summarized,including ESTs system,physical map construction,functional genomics and barley marker-assisted selection,which provides a theoretical basis for further improving molecular breeding and expounding the structural and functional performance of barley genome.