The Study on the Role of Intestinal Microecology Protecting Tight Junction Protein Between the Epithelial Cells of Intestinal Mucosal Barrier in Rats with Trinitro-Benzen-Sulfonic Acid Induced Colitis

Objective To investigate the effect of intestinal microecology protectiong tight junction proetein between the epithelial cells of intestinal mucosal barrier in rats with trinitro-benzen-sulfonic acid(TNBS) induced colitis.Methods Acute colitis was induced by giving TNBS.28 rats including male and female were randomized into normal group(n=8),TNBS group(n=10) and live combined bifidobacterium and lactobacillus tables(golden bific) group(n=10).Diarrhea and bloody stool as well as colonic histology were observed.The levels of tumor necrosis factor-α(TNF-α) and interleukin-10(IL-10) in colonic tissue and endotoxin in blood were determined with ELISA.The expression of occludin between the epithelial cells of intestinal mucosal barrier was detected by histochemical stain method.Results The inflammatory symptoms and histological damages of colonic mucosa in golden bifid group were better than those in TNBS group,but were worse than those in normal group.Compared with TNBS group,the levels of TNF-α and endotoxin in blood were significantly reduced(P0.05);the level of IL-10 and the expression of occludin between the epithelial cells of intestinal mucosal barrier were remarkably elevated(P0.05).Conclusion The data suggests that intestinal microecology has the ability of protecting tight junction protein between the epithelial cells of intestinal mucosal barrier through the mechanism of activating occludin protein,increasing the production of IL-10 and reducing the level of TNF-α and endotoxin in blood.