Objective To understand the effect of second-hand smoke to blood pressure,heart rate and respiratory frequency of rats,and provide the basis in revealing the harm of second-hand smoke to cardiovascular system and respiratory system.Methods Before and after exposing to second-hand smoke,heart rate and blood pressure of rats were measured using noninvasive blood pressure instrument,and their respiratory frequency by naked-eye respectively.Results After inhaling second-hand smoke,No.1 and No.2 of rats had accelerated heart rate and respiratory frequency,at the same time lowered blood pressure;rat No.3 had accelerated heart rate,decreased respiratory rate and blood pressure;rat No.4 had decreased heart rate,respiratory rate and blood pressure.Conclusion When the rats inhale second-hand smoke,blood pressure can decrease,and respiratory rate speed up within 25 minutes,but slow down after 25 minutes;meanwhile,heart rate speed up within 35 minutes,but slow down after 35 minutes.