161Actinomycetes strains were isolated from rhizosphere of wheat and soil.Seven strains was screened out from them,which have effective inhibitiion on the growth of pathogen(Rhizoctonia cerealis).However,the strain S 024 is the best one on inhibiting pathogen with the78.6%mycelia growth decrease.The effect of fermenting filtrate of S 024 on controlling disease was test in pots and plots.The results showed that the fermenting filtrate of S 024 can control wheat sharp eyespot effectively,with45.38%disease index decline in pots and63.21%decline plots.On the other hand,the fermenting filtrate of S 024 can increase germination rate of wheat seed,and promote the growth of seedling.