Analytical Methods for Determination of Reactive Oxygen Species

This review focuses on analytical methods for the determination of reactive oxygen species (ROS): a superoxide/ hydroperoxyl radical, hydrogen peroxide, singlet oxygen and a hydroxyl radical. The methods which are described in this paper are based on: electron paramagnetic resonance spectroscopy (EPR), nuclear magnetic resonance (NMR), mass spectroscopy (MS), spectroscopy UV-Vis, gas chromatography (GC), chemiluminescence (CL), fluorescence (FL), HPLC –UV, iodide titration, and 14C-formate oxidation. In this review, the most common analytical methods for the detection of ROS generated by chemical methods are presented. The methods are based on the direct as well as indirect detection of ROS. Keywords: Analytical methods, reactive oxygen species, radicals.