Development of Tolerance Induced by Oral Administration of Whey Hydrolysates in a Mouse Model of Cow’s Milk Allergy

No solution has been found to induce immune tolerance (IT) in patients with cow's milk allergy (CMA), and it is an urgent problem to resolve. In this study, the ability of different whey hydrolysates to induce IT was investigated based on the mouse model. Compared with the allergy group, the anaphylactic symptoms of three whey hydrolysate groups were significantly relieved. The levels of IgE and mMCP-1 were significantly reduced, and IL-4/5/13 and IL-6 cytokines secreted by spleen cells were significantly decreased. In addition, Th2 and Th17 cells were significantly downregulated and regulatory T cells were significantly upregulated. The CD103⁺DCs in mesenteric lymph node cells were significantly increased. Moreover, the cecum contents showed that the composition of the intestinal flora was significantly changed. This indicated that whey hydrolysates could induce the occurrence of IT. The study provides a theoretical basis and research direction to develop specific hydrolyzed products for allergic patients.