In order to improve the activation effect of deactivation catalysts, calcination of V 2 O 5 -WO 3 -MoO 3 /TiO 2 catalysts were investigated. The experimental results demonstrate that calcination time have an effect for recovering the activity of V 2 O 5 -WO 3 -MoO 3 /TiO 2 catalyst. Calcination not only ensures the better mechanical strength and integrity of the catalyst, but also plays an important role in the denigration activity of the catalyst. However, the particles of the catalyst surface enlarged with the increase of the calcination temperature. The suitable calcination time can improve the denigration efficiency and increase the mechanical strength of the catalyst. In addition, the calcination time mainly affects the formation of MoO 3 . These findings can provide reference for the activation of SCR-Den Ox catalyst.